Hemolytic Assay for Venom Phospholipase A
نویسندگان
چکیده
A rapid and sensitive spectrophotometric assay for venom phospholipase A2 based on the hemolysis of guinea pig erythrocytes in the presence of decomplemented serum and cardiotoxin (direct lytic factor) is described. This assay is particularly useful for rapid multisample analyses, such as those used in monitoring chromatography fractions, and is specific for phospholipase A2 in the presence of other potentially hemolytic venom components. The hemolytic mechanism is shown to be a combination of the action of lysophospholipids liberated from lipoproteins in the serum and the synergistic action of phospholipase A2 and cardiotoxin on the erythrocyte membrane.
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تاریخ انتشار 2001